DNA Extraction and Purification Process. Deoxyribonucleic Acid, commonly referred to as DNA, is the molecule found in everything termed as living. The DNA molecule contains a genetic code that is for every organism, therefore, acting as a blueprint for continuous...<br /><a class="read-more-button" href="http://yych.info/a-simple-plan-for-investigating-sequencing.html">Read more</a>
DNA Extraction and Purification Process.
Deoxyribonucleic Acid, commonly referred to as DNA, is the molecule found in everything termed as living. The DNA molecule contains a genetic code that is for every organism, therefore, acting as a blueprint for continuous plans for any substance that the organisms do produce in the course of their lives. Interestingly, as popular as the term DNA appears to be, very few people have had a chance to see the molecule simply because the molecule happens to be very small for one to easily see. However, one can carry out easy experiments right at home or in school using some household materials to extract DNA molecules even from plants like peas.
Some of the crucial materials that you may require for the process include alcohol, meat tenderizer, dishwashing detergent mostly in liquid form, a blender, a beaker or rather clear glass container, a stirring rod or a spoon. In the first procedure, blend at least a half cup of split peas in a cupful of water. With that, you should obtain a soupy mixture then use a homogenous solution between the mixtures. You may as well consider sparing some of the mixtures just in case. Secondly, pour some of the solution probably an adequate amount into a container up to a level of at least two inches.
The third step involves adding at least a single spoonful of the liquid dishwashing detergent on top of the solution. Again stir the mixture slowly for about five minutes. In the next procedure; add a pinch of the meat tenderizer on the mixture then stir for another five minutes. Having stirred the mixture, and then drizzle some alcohol above it. Consider adding the alcohol until you obtain a layer of three inches on the split peas solution. Peas are particularly considered during the extraction because of their high source of this molecule. The use of the detergent is to break down the membrane of the cell hence making it release the contents and the meat tenderizer to dissolve the membrane of the nuclear in order to make it release the DNA inside. The alcohol is used in the process to bring out the DNA strands.
The purification procedure can utilize various processing kits. Different samples work with different kind of tissues, plant parts, forensic samples as well as cellular structures. On a daily basis, there are new inventions of machines and cutting edge products through various forms of amplification and facilitations for use in new projects and experiments. Among these ways are the use of magnetic beads and the way happens to be very effective. Another way involves the use of plasmid purification kits which is able to give low endotoxin levels for use in the subject transfection.